Authors

Abstract

In this study, the effects of different electric field parameters on the germination characteristics of aged rice seeds were determined via low-frequency high-voltage pulsed electric field (LH-PEF) treatment. The Design-Expert software was used to design a rotating combination test, and response surface analysis was used to optimize five germination characteristics of rice seeds. The optimal treatment voltage was 13 kV and the optimal treatment time was 32.72 min. The results show that, under the optimal treatment conditions (P<0.01), the germination potential (GE), germination rate (GP), germination index (GI), vitality index (VI) and high vitality ratio (HVR) of the aged rice seeds increased by 23.7%, 17.7%, 43.2%, 59.7%, and 250%, respectively, compared to the control. The pulsed electric field’s biological effect on the aged rice seeds was the most significant when the optimal treatment parameters were used. The results of this study provide a reliable reference for the parameter optimization of high-voltage electric field treatment of crop seeds.

Abstract in Chinese

本研究通过低频高压脉冲电场(LH-PEF)处理陈年水稻种子，研究不同电场参数对其萌发特性的影响，为提高陈年水稻种子活力提供了一种物理方法。本文利用Design-expert软件设计旋转组合试验，并利用响应面分析法，对水稻种子的5个萌发特性指标进行参数优化，得出最优处理电压为13kV，最优处理时间为32.72min。结果表明：陈年水稻种子在最优处理条件下，与对照相比，发芽势(GE)提高了23.7%，发芽率(GP)提高了17.7%，发芽指数(GI)提高了43.2%，活力指数(VI)提高了59.7%，高活力比(HVR)提高了250%，各指标都达到极显著差异（P<0.01）。在最优参数的脉冲电场作用下，陈年水稻种子的电场生物学效应最明显，研究结果为后续作物种子高压电场处理的参数优化提供参考。