Authors

Abstract

PEF pretreatment enhances Scutellaria seed germination and growth, improving drought adaptability. Using PEG-6000 to simulate drought stress, germination potential (42.86%), germination rate (44.76%), and germination index (27.73%) were significantly improved under 12.5% PEG. PEF treatment also increased vigor index, root length, and dry weight, shortened germination time, enhanced SOD and POD activities, reduced MDA content, and elevated levels of soluble sugars, soluble proteins, proline, and α-amylase. Hormonal analysis revealed increased gibberellin and auxin contents accompanied by reduced ABA levels. Overall, PEF pretreatment effectively promoted seed germination and growth under drought stress conditions.

Abstract in Chinese

PEF预处理黄芩种子，可促进发芽和幼苗生长，有利于适应和抵抗不利生长环境。本文以PEG-6000模拟干旱胁迫，观测有、无PEF处理的黄芩种子在不同干旱胁迫下的萌发和生长状况，探讨PEF处理提升黄芩种子抗旱性的生理机制。结果显示：12.5% PEG胁迫下，PEF处理可以使黄芩种子的发芽势、发芽率、发芽指数分别显著提高42.86%、44.76%和27.73%；活力指数、胚根长、干重增加，平均发芽时间缩短。PEF处理使胁迫下生长的幼苗SOD和 POD酶活性提高20.13%-40.13%和40.00%-52.69%，丙二醛含量下降35.44%-43.74%，可溶性糖、可溶性蛋白和脯氨酸含量增加28.57%-39.13%、28.41%-79.07%和29.32%-80.07%，α-淀粉酶活性增加15.91%-53.74%；赤霉素和生长素含量在萌发和幼苗期升高4.06%-23.06%和16.62%-26.89%，脱落酸含量下降20.50%-25.45%。因此，PEF预处理黄芩种子能保持干旱胁迫下种子萌发和生长的顺利进行。