Authors

Abstract

Aiming to address the scarcity of semi-continuous experimental studies on chicken manure (CM) and corn stover (CS), as well as the unclear patterns of microbial community changes during digestion, this research employs both sequential batch and semi-continuous experimental methods for investigation. Sequential batch experiments showed that the ACoD of CM and CS had the highest cumulative methane yield (82.45(±0.80) mL/g VS), high Rm and small λ, while the ammonia nitrogen (NH4+-N) concentration generated during the reaction process was below the threshold of inhibitory concentration, indicating that CM and CS were more suitable as an anaerobic digestion (AD) substrate. The semi-continuous experiments showed that the maximum total daily gas production rate of 649.15 mL/gVS was achieved at an organic load rate (OLR) of 0.31 gVS/(L·d). NH4+-N concentration up to 4214.5 mg/L inhibited the semi-continuous flow digestion system. Microbial analysis showed that Firmicutes, Bacteroidota, and Proteobacteria were the dominant phyla throughout the AD experiment. Sedimentibacter, Breznakibacter, Methanosarcina, and Methanobacterium were significantly and positively correlated with methane production and improved the performance of CM treatment with CS. This study analysed the semi-continuous AD of CM and CS under different OLR, which can provide a reference for engineering applications.

Abstract in Chinese

为解决鸡粪与玉米秸秆连续实验研究匮乏及消化过程中微生物群落变化规律不明晰的问题，本研究采用序批式与半连续实验方法进行联合探究。序批式实验表明，鸡粪和玉米秸秆共消化具有最高的累计甲烷产率(82.45(±0.80) mL/g VS)、较高的甲烷生产速率和较小的滞后期。同时反应过程产生的氨氮浓度处于抑制浓度阈值以下,表明鸡粪和玉米秸秆更适合作为厌氧消化底物。半连续实验表明，当有机负荷率为0.31g VS/(L·d) 时，日总产气量最高，达到649.15 mL/gVS。氨氮浓度高达4214.5 mg/L时，半连续流消化系统受到抑制。微生物分析表明，在整个实验过程中，Firmicutes、Bacteroidota、Proteobacteria是优势菌门。Sedimentibacter、Breznakibacter、Methanosarcina和Methanobacterium与甲烷产量呈显著正相关关系，并提高了鸡粪和玉米秸秆的处理效率。本研究分析了不同有机负荷率下鸡粪和玉米秸秆的半连续厌氧消化过程，为工程应用提供了参考依据。